HMRC decides using the tax return you submitted for an earlier year: 2024/25 income decides whether you start in April 2026, 2025/26 income decides April 2027 and 2026/27 income decides April 2028. If you run more than one trade, the turnovers are added together.
Income from employment, dividends and pensions does not count. Your share of partnership profit does not count either for now, because HMRC has said partnerships will join later on a timeline still to be announced. The test is "over" the limit, so exactly the threshold is not caught.
The £1,000 trading allowance does not reduce qualifying income. The regulations take each amount before any deductions, and our checker follows that. If you are close to a limit, confirm with HMRC before relying on any estimate, ours included.